Gene Cloning: vectors, plasmids, ligations, and transformation - oh my!
Posted in Techniques Examined • Tagged with Introductory, Detailed
The goal of gene cloning is to get a single copy of the gene from an animal and into an archive. The rest is just the individual steps necessary to do this. Cloning genes allows us to easily and consistently retrieve the exact same copy and to share it between labs or even organisms.
|1||Get a copy of the gene from the animal||PCR|
|2||Put the gene in an archival format||Ligation into a plasmid|
|3||Make backup copies or archive it||Bacterial transformation|
|4||Confirm that the copies match the original||Sequence the insert|
|5||Retrieve a copy to use||Miniprep|
What is a vector and what how is it used?
A vector is a small, self contained piece of DNA that can be added to an organism, typically bacteria. It is how we archive the gene we are cloning.Continue reading