Gene Cloning: vectors, plasmids, ligations, and transformation - oh my!
Posted in Techniques Examined • Tagged with Introductory, Detailed
The goal of gene cloning is to get a single copy of the gene from an animal and into an archive. The rest is just the individual steps necessary to do this. Cloning genes allows us to easily and consistently retrieve the exact same copy and to share it between labs or even organisms.
| Step | Description | Biology Jargon | |
|---|---|---|---|
| 1 | Get a copy of the gene from the animal | PCR | |
| 2 | Put the gene in an archival format | Ligation into a plasmid | |
| 3 | Make backup copies or archive it | Bacterial transformation … |