Aug 10, 2016
What is the goal or what is gene cloning?
The goal is to get a single copy of the gene from an animal and into an archive. The rest is just the individual steps necessary to do this.
||Get a copy of the gene from the animal
||Put the gene in an archival format
||Ligation into a plasmid
||Make backup copies or archive it
||Confirm that the copies match the original
||Sequence the insert
||Retrieve a copy to use
What is a vector?
A vector is a small, self contained piece of DNA that can be added to an organism, typically bacteria.
Aug 09, 2016
Genes and Phenotype
We are constantly seeing both news articles as well as scientific publications on new genes - the new gene for intelligence, cancer or happiness. So lets start at the beginning by figuring out what a gene is. Simply, its a way to refer to inherited traits.
There are a wide variety of traits that are controlled by genes. Flower color is a pretty obvious trait, so lets use a flower that comes in both white and red as an example. We call the specific ...
All of biology functions around the core of DNA, RNA and Proteins or the so called Central Dogma of Molecular Biology. The key here is that you always go in the order of DNA→RNA→protein. You can’t jump from DNA to protein or visa versa; it just doesn’t happen. However, you don’t have to go all the way. Just because you made RNA doesn’t automatically mean it will be a protein. This is also not necessarily a one-to-one relationship. One RNA can be used to make several copies of the exact same protein.
Why do I care?
Because a gene can be looked at in each step of the DNA→RNA→protein progression. We look at all the different steps because how a gene is functioning can be regulated or changed at any point. However, in all cases, we are still interested in the gene..
Biological Levels explained through the anology of a book.