What is the goal or what is gene cloning?
The goal is to get a single copy of the gene from an animal and into an archive. The rest is just the individual steps necessary to do this.
|1||Get a copy of the gene from the animal||PCR|
|2||Put the gene in an archival format||Ligation into a plasmid|
|3||Make backup copies or archive it||Bacterial transformation|
|4||Confirm that the copies match the original||Sequence the insert|
|5||Retrieve a copy to use||Miniprep|
What is a vector?
A vector is a small, self contained piece of DNA that can be added to an organism, typically bacteria.