Biology Fun-damentals

Aug 10, 2016

Gene Cloning: vectors, plasmids, ligations, and transformation - oh my!

What is the goal or what is gene cloning?
The goal is to get a single copy of the gene from an animal and into an archive. The rest is just the individual steps necessary to do this.

Step Description Biology Jargon
1     Get a copy of the gene from the animal PCR
2     Put the gene in an archival format Ligation into a plasmid
3     Make backup copies or archive it Bacterial transformation
4     Confirm that the copies match the original    Sequence the insert
5     Retrieve a copy to use Miniprep

What is a vector?
A vector is a small, self contained piece of DNA that can be added to an organism, typically bacteria.

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